Review of COVID-19 Paper Methods: Sequencing the "Virus"

In this video I go over the main COVID-19 "virus" isolation paper's methods on the techniques used in sequencing this "new virus". The first step in sequencing the "virus" is assuming that the RNA found in the patient airway fluid samples and cell culture supernatants somehow belong to a "virus". After this assumption, a combination of Illumina sequencing and nanopore sequencing were used to somehow produce a "virus genome". After this, PCR tests were designed with specific primers to test for this new "virus". The "virus" sequences were then compared with reference sequences. And finally, statistical analysis was performed on determining the likely evolutionary history of the "virus" genome. Essentially, the paper outlines a lot of obscure computer and statistical techniques to form long genetic sequences from RNA assumed to be from a "virus". Truly bizarre "science"...

The paper is listed below:

• A Novel Coronavirus from Patients with Pneumonia in China, 2019
• Authors: Na Zhu et al
• Published on January 24, 2020 in The New England Journal of Medicine (NEJM)
• https://www.nejm.org/doi/full/10.1056/NEJMoa2001017

The timestamps of key parts of the video are listed below:

• Paper Methods: Sequencing the "Virus": 0:00
• RNA as a Template to Clone and Sequence Genome: 0:37
• Illumina Sequencing, Nanopore Sequencing: 1:13
• Sequence Reads Assembled into Contig Maps: 1:46
• Contig, Contiguous, Contig Map: 2:00
• Primers Designed for PCR to Fill Genome Gaps: 3:11
• Primer, 5' or 3', Complementary DNA (cDNA), Sanger Sequencing: 3:44
• Multiple-Sequence Alignment of New "Virus": 6:19
• Multiple-Sequence Alignment, Phylogenetics, Bootstrap Replicates: 6:41
• Summary of Sequencing the "Virus": 7:30

This video is taken from my earlier video listed below:

• 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8
• Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper
• Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx

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