Review of Paper Methods: Detecting "Viruses" (Main Paper on COVID-19 "Virus" Isolation)

In this video I go over a review of the "Viral" Diagnostic Methods from the main paper on COVID-19 "virus" isolation. The paper states that 4 lower respiratory tract samples were collected from Wuhan pneumonia patients (of "unknown cause") in December 2019 and were compared with 7 samples from Beijing pneumonia patients (of "known cause"), yet this comparison is never properly detailed in the paper. Nucleic acids were extracted from the samples and then polymerase chain reaction (PCR) was used to test for "22 known viruses and 4 known bacteria". Next generation sequencing (NGS) technology was then performed and they somehow detected sequences not discovered by the PCR tests. Reverse transcription PCR (RT-PCR) was then used to detect "viral RNA", which later in the paper they conclude is from a novel "coronavirus". This appears more as an exercise in computer generated sequencing than actual detection of real-life "disease causing particles"...

The paper is listed below:

• A Novel Coronavirus from Patients with Pneumonia in China, 2019
• Authors: Na Zhu et al
• Published on January 24, 2020 in The New England Journal of Medicine (NEJM)
• https://www.nejm.org/doi/full/10.1056/NEJMoa2001017

The timestamps of key parts of the video are listed below:

• Viral Diagnostic Methods: 0:00
• Respiratory Tract, Bronchoalveolar-lavage : 1:14
• Extraction of Nucleic Acids: 2:02
• Cell Cultures, Negative Controls: 2:36
• PCR to Detect "Viruses" and Bacteria: 3:17
• Polymerase Chain Reaction (PCR), PCR cycles, dNTPs, Primers: 3:57
• NGS to Detect New Sequences, RT-PCR to detect "viral" RNA: 6:47
• Unbiased High-Throughput Sequencing or Next Generation Sequencing (NGS) 7:20
• Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR): 8:40
• Pan B-CoV, RNA-dependent RNA polymerase (RdRp): 9:40
• Summary of Detecting the "Virus": 11:25

This video is taken from my earlier video listed below:

• 🔥#MESScience 4: Review of COVID-19 "Virus" Isolation Paper: https://youtu.be/cvDO85Rw4d8
• Video notes: https://peakd.com/hive-128780/@mes/messcience-4-review-of-covid-19-virus-isolation-paper
• Playlist: https://www.youtube.com/playlist?list=PLai3U8-WIK0E4aQ_cq4ZDD2WGiDU5vVgx

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